A simple method for sequencing the complementary strand of ssDNA from M13 clones

A simple and rapid preparation of M13 sequencing templates for manual and automated dideoxy sequencing.

A simple and rapid procedure for the preparation of M13 single stranded DNA sequencing templates which does not involve phenol extractions and alcohol precipitations is described. Bacteriophages are precipitated from media supernatants with acetic acid and recovered on glass fiber filters. Subsequent dissociation of the phages and removal of contaminants is performed while the DNA is bound to t...

A simple method for the determination of serum cholesterol

investigating the feasibility of a proposed model for geometric design of deployable arch structures

deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...

from linguistics to literature: a linguistic approach to the study of linguistic deviations in the turkish divan of shahriar

chapter i provides an overview of structural linguistics and touches upon the saussurean dichotomies with the final goal of exploring their relevance to the stylistic studies of literature. to provide evidence for the singificance of the study, chapter ii deals with the controversial issue of linguistics and literature, and presents opposing views which, at the same time, have been central to t...

A simple method for screening of NotI linking clones.

Notl linking clones are valuable for ordering of OW fragnents fractionated by Pulsed f ield gel electrophoresis(l) and especially Interested in that they are often associated with transcribable genes(2,3). To faci l i tate the isolation of Notl linking clones, we have devised a method which based on the insertion of Kanamycin cassette bearing Notl sites at both ends into cleavable Notl sites of...